Video presented as part of the CDI-Roche webinar December 14, 2011.
This video is courtesy of and copyrighted by Roche, Inc.
Ion channel block is detected as irregular contractile activity in the hCAR Assay. iCell Cardiomyocytes were plated as a confluent monolayer and exposed to 0.03 mM of the hERG channel blocking agent, E-4031. The video shows the rhythmic contractile activity of the cardiomyocytes under control conditions (pre-drug) and the arrhythmic activity following drug application (E-4031 0.03 mM). The data tracings shown below illustrate the data obtained with the hCAR assay on the xCELLigence RTCA Cardio System. The top tracing shows the rhythmic activity prior to drug application and the bottom tracings show the arrhythmic activity post-drug application. The ectopic activity observed in the video manifests as an altered beat rate (green circles) and smaller, arrhythmic beats (red arrows).
