iCell Cardiomyocytes

iCell® Prototypes

Cellular Dynamics is committed to enabling the research community with access to relevant human biology through the rapid introduction of novel iPS cell-derived tissue cells. To this end, CDI is engaged in the development of a variety of novel human iCell® prototypes.  The iCell prototypes listed below are commercially available today. Looking for another cell type?  Contact us for more information. 

iCell Astrocytes

iCell Astrocytes are a heterogeneous population of human astrocytes derived from iPS cells. Astrocytes, the most abundant cell type within the CNS, are responsible for a wide variety of complex and essential functions in the CNS. In addition, the loss of or changes in normal astrocyte functions play a critical role in neurodegenerative diseases, including Alzheimer's and Parkinson's diseases, as well as other neurological disorders. iCell Astrocytes provide a reliable source of high quality, high purity human astrocytes and are ideal for in vitro astrocyte-mediated neurotoxicity, targeted drug discovery, blood brain barrier modeling, and other life science research.

iCell Astrocytes express S100 calcium binding protein B (S100Β) and glial fibrillary acidic protein (GFAP) and secrete trophic factors with stimulation. These cells are shipped frozen as cryopreserved cells. Contact us for more information.

 
 

iCell Astrocytes
A highly pure astrocyte population demonstrating typical morphology (top) and expression of GFAP (bottom).

 
   

Literature

iCell Astrocytes Datasheet
(PDF)

For More Information

We are actively seeking collaborative partnerships to develop this cell type and assays, and we welcome inquiries.

Email us or contact Cellular Dynamics:
+1 (608) 310-5100 | US toll-free (877) 310-6688

iCell Hematopoietic Progenitor Cells

Derived from human iPS cells, iCell Hematopoietic Progenitor Cells are human multipotent hematopoietic progenitor cells that can be further differentiated into diverse terminal cell types within the hematopoietic lineage. The capacity to generate and provide highly purified human hematopoietic progenitor cells benefits multiple areas of biological research including basic hematopoiesis, cellular therapy, cancer immunotherapy, induced transplant tolerance, treatment for autoimmune diseases, and toxicity testing.

iCell Hematopoietic Cells are provided as a cyropreserved highly pure CD34+ population. Contact us for more information.



iCell Hematopoietic Progenitor Cells
iCell Hematopoietic Progenitor Cells are multipotent precursor cells that give rise to a variety of hematopoietic cell types.

 

Multipotency of iCell Hematopoietic Progenitor Cells
The multipotency of iCell Hematopoietic Progenitor Cells is demonstrated by their performance in colony-forming unit (CFU) assays. Note the presence of cellular processes in the CFU-MK assay indicating functional megakaryocytes.

 
   

Literature

iCell Hematopoietic Progenitor Cells Datasheet
(PDF)

For More Information

We are actively seeking collaborative partnerships to develop this cell type and assays, and we welcome inquiries.

Email us or contact Cellular Dynamics:
+1 (608) 310-5100 | US toll-free (877) 310-6688

iCell Cardiac Progenitor Cells

Cellular Dynamics International (CDI) offers iCell Cardiac Progenitor Cells, the only commercially available, truly human cardiac progenitor. Derived from human induced pluripotent stem (iPS) cells, iCell Cardiac Progenitor Cells are a population of dynamic progenitors that are able to differentiate into terminally derived cardiac cell types, thus providing an in vitro doorway into native developmental cardiac biology.

Cardiac progenitor cells (CPCs) represent the earliest stages of mesodermal commitment to the cardiac lineage. iCell Cardiac Progenitor Cells show a classical CPC marker profile of KDR/PDGFR-αpos/CKITneg and are responsive to permissive conditions for proliferation as a progenitor population and/or differentiation into terminal cardiac cell types. Such in vitro recapitulation of native biology provides a powerful tool for understanding cardiac development and regeneration while enabling therapeutic research programs directed against cardiac ailments and disease states that have been difficult, if not impossible, to investigate with relevant human material.

CPC Flow Cytometry

iCell Cardiac Progenitor Cells Provide Relevant Biology
(A) Flow cytometry measurements of iCell Cardiac Progenitor Cells as a KDR/PDGFR-αpos/CKITneg cell population. (B) Immunocytochemical staining and flow cytometry analysis of iCell Cardiac Progenitor Cells before (bottom) and after (top) differentiation into cardiomyocytes.

CPCs are traditionally accessed through labor-intensive isolations from non-human primary tissue sources. Such isolations typically yield small numbers of true progenitors, suffer from source availability and variability, and ultimately must be translated to the human condition. iCell Cardiac Progenitor Cells are derived in industrial volumes through controlled conditions from a single population of human iPS cells, thereby circumventing sourcing, variability, and species-related limitations.

The human origin, high volume, high quality, and robust nature of iCell Cardiac Progenitor Cells provide a relevant substrate for traditional small molecule/biologic discovery screens, as well as a platform to develop methods whereby progenitor cells can be used as a regenerative medicine therapeutic.

Contact us for more information.

Specifications

Cell Type

Cardiac progenitors

Catalog #

CPC-301-020-001-PT

Organism

Human

Source

Differentiated from a CDI-derived human iPS cell line

Quantity

>5M viable cells/unit

Shipped

Frozen

Storage

Liquid nitrogen

Media

None

Advantages

Human Cells

Cardiac developmental programs are species-specific. Traditional models are based on non-human, typically rodent, models, and must use underlying assumptions when translating to the human condition. iCell Cardiac Progenitor Cells are derived from a human iPS cell source and thus avoid difficulties associated with species-specific biology.

Relevant Biology

Native development from blastula to cardiac tissue involves progressive commitment and restriction to particular tissue types. iCell Cardiac Progenitor Cells recapitulate this progression, arising from the pluripotent stem cell population by differentiating through mesodermal and cardiac mesodermal stages to the myocardial progenitor stage. Upon thaw, they can be cultured under permissive conditions to terminal cardiac cell types, such as functional cardiomyocytes, thus providing a window to native human cardiac developmental biology.

HTS Ready

iCell Cardiac Progenitor Cells are provided as cryopreserved suspensions of single cells and can be cultured in 96- and 384 well plates at the end-user's convenience, thus providing a human-based solution for targeted and phenotypic screens.

Unlimited Supply

Restoring normal cardiac tissue and function to individuals recovering from myocardial infarction(s) or suffering from congestive heart failure is an area of intense research in drug development and regenerative medicine. These efforts have been hampered by a lack of reliable, consistently available, and relevant models. iCell Cardiac Progenitors overcome these problems and provide researchers with an inexhaustible supply of relevant human biological material for basic and targeted research into cardiac development and regeneration.

Applications

iCell Cardiac Progenitor Cells are amenable to use on multiple platforms to study a variety of cellular functions and assay endpoints including:

  • Developmental toxicity
  • Differentiation competency
  • Proliferative capacity

HTS Reproducibility

iCell Cardiac Progenitor Cells Provide Robust Data
Proliferation and differentiation assays in 96-well plates with iCell Cardiac Progenitor Cells across multiple manufacturing batches provide robust and repeatable results with Z’ scores suitable for HTS screening.


To Order

Request a quote or contact Cellular Dynamics:
+1 (608) 310-5100 | US toll-free +1 (877) 310-6688

In Japan, contact IPS Academia Japan:
+81 75 256-8582

 

Units
per Vial

Catalog #

iCell Cardiac Progenitor Cells

1

 CPC-301-020-001-PT


Support

Training

To receive advanced, in-laboratory education on the use of iCell products, inquire about CDI's iCertification Training Programs.

Frequently Asked Questions

See the list of FAQs here.

Technical Support

Call +1 (608) 310-5100 | US toll-free (877) 310-6688 or submit your technical question online.

Literature

Reference Materials

Datasheet

iCell Cardiac Progenitor Cells
(PDF)

Technology Overview

Datasheet

Cellular Dynamics International: True Human Biology in a Dish
(PDF)

Poster

Reprogramming Human Peripheral Blood Cells
(PDF, presented at ISSCR 2009)

Bibliography

Published Research
(HTML)

iCell Skeletal Myoblasts

Cellular Dynamics provides iCell Skeletal Myoblasts, human induced pluripotent stem cell (iPSC)-derived skeletal myoblasts. These human myoblasts deliver in vitro access to native human skeletal myoblast and myotube biology, providing a relevant human substrate for basic research work, targeted mechanistic investigations, drug discovery, and regenerative medicine.

Derived from human iPSCs, iCell Skeletal Myoblasts are mesodermally commited skeletal myoblasts that fuse to form multi-nucleated functional skeletal myotubes. The capacity to generate and provide highly purified human skeletal myoblasts from a single isogenic source provides investigators powerful access to robust and repeatable native skeletal muscle cell biology with all the benefits of a robust in vitro tool. iCell Skeletal Myoblasts cells can be used to understand and modulate basic cellular developmental processes, implemented into targeted and phenotypic screens for molecular therapies, and be deployed as a platform to develop cellular therapies for regenerative medicine.

   
Skeletal Myoblast Flow Diagram
MYH7 Positive iCell SKM

iCell Skeletal Myoblasts
A highly pure skeletal myoblast population as demonstrated by Troponin T (flow diagram, top) and MYH7 positive fluorescent immunostaining (micrograph, bottom).

 
   

Specifications

Cell Type

Skeletal Myoblasts

Catalog #

SKM-301-020-001-PT

Package Size

1 unit per vial

Quantity

>8M viable, adherent cells/unit

Organism

Human

Sub-type

Myogenin and MYH7 positive

Source

Differentiated from a CDI-derived human iPS cell line

Growth Properties

Adherent

Shipped

Frozen

Storage

Liquid nitrogen

Media

None

Advantages

Human Cells

Skeletal muscle cell biology can be species-specific.  Traditional models are based on non-human, typically rodent, models and must use underlying assumptions when translating to the human condition. iCell Skeletal Myoblasts are derived from a single isogenic human iPSC source and thus avoid difficulties associated with species-specific biology.

Relevant Biology

Native development progresses from a mesodermally committed cell population to mononucleated myoblasts to polynucleated mechanically active myotubes through cellular fusion.  iCell Skeletal Myotubes recapitulate this progression; arising from the pluripotent stem cell population by differentiating through mesodermal stages to skeletal myoblasts to skeletal myotubes.  Upon thaw they rapidly fuse to form polynucleated myotubes that are mechanically active, thus providing a window to native human skeletal muscle cell biology.

Laboratory Ready

iCell Skeletal Myoblasts are provided as cryopreserved suspensions of single cells and can be cultured in 96 well plates at the end-users convenience. iCell Skeletal Myoblasts are cultured in a single media; expansion, differentiation and culture in multiple medias is unnecessary thus making handling easier compared to primary preparations. Myotubes are generated from a single isogenic iPSC source and thus exhibit minimal variability in function and are highly reproducible. Functional studies examining electrical, biochemical, and mechanical functionality can be implemented to provide human-based solution for basic research as well as targeted and phenotypic screens.

Applications

iCell Skeletal Myoblasts have demonstrated utility in biochemical assays for use in drug discovery, regenerative medicine, and basic life science research.


HTS Reproducibility
Robust and Repeatable

iCell Skeletal Myoblasts Provide Robust Data
AKT phosphorylation and de-phosphorylation in response to IGF-1 or myostatin, respectively. IGF-1 phosphorylation is robust and repeatable across manufacturing batches.

Literature

Technology Overview

Datasheet

Cellular Dynamics International: True Human Biology in a Dish
(PDF)

Poster

Reprogramming Human Peripheral Blood Cells
(PDF, presented at ISSCR 2009)

Bibliography

Published Research
(HTML)

To Order

Request a quote or contact Cellular Dynamics:
+1 (608) 310-5100 | US toll-free +1 (877) 310-6688

In Japan, contact IPS Academia Japan:
+81 75 256-8582

 

Units
per Vial

Catalog #

iCell Skeletal Myoblasts

1

 SKM-301-020-001-PT





Support

Training

To receive advanced, in-laboratory education on the use of iCell products, inquire about CDI's iCertification Training Programs.

Frequently Asked Questions

See the list of FAQs here.

Technical Support

Call +1 (608) 310-5100 | US toll-free (877) 310-6688 or submit your technical question online.