CDI develops and manufactures human cells derived from induced pluripotent stem (iPS) cells.  CDI's product portfolio includes:

• iCell Cardiomyocytes
• iCell Endothelial Cells
• iCell Neurons
• iCell Hepatocytes (coming in 2012)
• MyCell Products

CDI's iCell products offer numerous advantages over existing cellular models.  Specifically, CDI's iCell products are:

• Biologically relevant human cells that exhibit characteristic phenotypes and functions.
• Highly pure (>95%) populations of human cells
• Readily available in the quantities required for use in drug discovery and safety testing.
• Supported with the necessary media, protocols, training and technical/application support to ensure customer success).

No specialized training is needed to use CDI's iCell products. However, it is critical that CDI's iCell products be handled as described in the User's Guides. In addition, CDI offers training videos, technical/application support, and hands-on iCell Certification Programs to ensure customer success.

iCell Cardiomyocytes are a pan-cardiac population of ventricular, atrial, and nodal cells.  Based on CDI's stringent criteria for classifying cardiac subtypes, iCell Cardiomyocytes consist of approximately 50% ventricular, 25% atrial, and 25% nodal cells.

iCell Cardiomyocytes are >95% pure.

iCell Cardiomyocytes are shipped cryopreserved to ensure maximum flexibility for use in a wide range of assays and applications.

iCell Cardiomyocytes are terminally differentiated cardiomyocytes and similar to native human cardiomyocytes, they do not proliferate.

iCell Cardiomyocytes are metabolically active and beat spontaneously in culture, enabling robust measurements of electrophysiology, contractility, energetics, and intracellular signaling in a species-specific manner using a variety of assays and platforms.

CDI has demonstrated stable cardiomyocyte gene expression for up to 120 days and has maintained stable, beating monolayers of iCell Cardiomyocytes for greater than 9 months.  iCell Cardiomyocytes are provided with enough media for two weeks of culture and additional Maintenance Media can be purchased separately for applications requiring longer-term culture.

iCell Cardiomyocytes have been extensively characterized over many physiological attributes and functions including (but not limited to):

• Morphology
• Gene and protein expression
• Electrophysiology
• Contractility
• Biochemical pathways
• Response to known cardiotoxicants and pathological insults

iCell Cardiomyocytes have demonstrated utility in a wide variety of  applications including (but not limited to):

• General cardiomyocyte biology
• Toxicology
• Safety pharmacology
• Drug discovery
• Disease research

• Perforated and whole cell manual patch clamp recording
• Voltage clamp recording
• Current clamp recording
• Automated patch clamp recording
• PatchXpress (Molecular Devices)
• Patchliner (Nanion)
• Qpatch (Sophion)
• Multi-Electrode Array (MEA) recording
• Multi-Channel Systems
• Axion MEA (Axion Biosystems)
• MED 64 (Alpha Med Scientific)
• Contractility/GPCR Screening/Ca2+ handling
• FLIPRTETRA GPCR and Ion Channel High Throughput Screening System (Molecular Devices)
• Myocyte calcium and contractility recording system (Ionoptix)
• Functional drug screening system (Hamamatsu)
• High Content Analysis
• ImageXpress (Molecular Devices)
• Cellomics ArrayScan VTI (Thermo Scientific)
• Label-Free Analysis
• xCELLigence RTCA Cardio System (ACEA/Roche Applied Science)
• Bioenergetics
• Seahorse Extracellular Flux (XF) Analyzer (Seahorse Bioscience)
• Cell Health and Viability Assays
• CellTiter-Glo, Caspase-Glo, CytoTox, MultiTox Assays, GSH-Glo, HDAC-Glo (Promega)
• Cell Meter JC-10 Mitochondrial Membrane Potential Assay (ABD Bioquest)
• CyQUANT Direct Cell Proliferation Assay (Invitrogen)

iCell Cardiomyocytes have been cited in numerous publications. Refer to the Published Research webpage.)

CDI provides extensive product literature and technical support tools to enable the use of iCell Cardiomyocytes across a broad range of applications. Refer to the Product Literature webpage for a product User's Guide, Application Notes, Datasheets, and a training video. Hands-on training through CDI's iCertification Program is also available. Additional assistance can be obtained by contacting Technical and Application Support by completing the online form or by calling +1 (608) 310-5100 or (877) 310-6688 (US toll-free).

iCell Endothelial Cells are >95% pure.

iCell Endothelial Cells are shipped cryopreserved to ensure maximum flexibility for use in a wide range of assays and applications.

Yes. iCell Endothelial Cells can be maintained in culture for up to 5 passages.

iCell Endothelial Cells have been characterized for the following:

• Morphology
• Gene and protein expression
• Tube formation
• Tight junction formation
• Barrier function
• Acetylated LDL uptake
• Response to inflammatory stimuli

iCell Endothelial Cells can be used for the following applications:

• Drug discovery
• Oncology research
• Inflammation research
• Cardiovascular disease research

iCell Endothelial Cells have been used in a number of assays and platforms including:

• Cell migration assays
• Electrical Cell-substrate Impedence Sensing (ECIS) System (Applied Biophysics)
• Barrier Function Assays
• xCELLigence RTCA Cardio System (ACEA/Roche Applied Science)
• Electrical Cell-substrate Impedence Sensing (ECIS) System (Applied Biophysics)
• Bioenergetics
• Seahorse Extracellular Flux (XF) Analyzer (Seahorse Bioscience)
• Angiogenesis Assays
• Endothelial Tube Formation Assay
• Electrical Cell-substrate Impedence Sensing (ECIS) System (Applied Biophysics)

CDI provides extensive product literature and technical support tools to enable the use of iCell Endothelial Cells. Refer to the Product Literature webpage for a product User's Guide, Datasheet, application data, and poster presentation. Additional assistance can be obtained by contacting Technical and Application Support by completing the online form or by calling +1 (608) 310-5100 or (877) 310-6688 (US toll-free).

iCell Neurons are a mixture of post-mitotic neural subtypes, comprised of approximately equal parts of GABAergic and glutamatergic neurons and absent of astrocytes as measured by gene and protein expression.

iCell Neurons are >95% pure.

iCell Neurons are shipped cryopreserved to ensure maximum flexibility for use in a wide range of assays and applications.

iCell Neurons are terminally differentiated neurons and similar to native human neuron. They do not proliferate.

iCell Neurons are metabolically active enabling robust measurements of electrophysiology, energetics, and intracellular signaling in a species-specific manner using a variety of assays and platforms.

iCell Neurons display physiological characteristics and responses and a stable adherent single-cell morphology for an extended culture period (>14 days). iCell Neurons are provided with enough media for more than two weeks of culture. Additional Maintenance Media can be purchased separately for applications requiring longer-term culture.

iCell Neurons have been extensively characterized over many physiological attributes and functions including (but not limited to):

• Morphology
• Gene and protein expression
• Electrophysiology
• Biochemical pathways
• Response to known neurotoxicants

iCell Neurons have demonstrated utility in a wide variety of  applications including (but not limited to):

• General neurobiology
• Toxicology
• Drug discovery
• Disease modeling

iCell Neurons have been used with a broad range of assays and platforms including (but not limited to):

• Perforated and whole cell manual patch clamp recording
• Voltage clamp recording
• Current clamp recording
• Automated patch clamp recording
• PatchXpress (Molecular Devices)
• Patchliner (Nanion)
• Multi-Electrode Array (MEA) recording
• Multi-Channel Systems
• High Content Analysis
• ImageXpress (Molecular Devices)
• Cell Health and Viability Assays
• CellTiter-Glo, Caspase-Glo, CytoTox, MultiTox Assays, GSH-Glo, HDAC-Glo (Promega)
• Cell Meter JC-10 Mitochondrial Membrane Potential Assay (ABD Bioquest)
• CyQUANT Direct Cell Proliferation Assay (Invitrogen)

CDI provides extensive product literature and technical support tools to enable the use of iCell Neurons across a broad range of applications. Refer to the Product Literature webpage for a product User's Guide, Datasheet, application data, and poster presentations. Additional assistance can be obtained by contacting Technical and Application Support by completing the online form or by calling +1 (608) 310-5100 or (877) 310-6688 (US toll-free).