iCell Cardiomyocytes

How can we help?

As the world’s largest producer of fully functional human cells derived from induced pluripotent stem (iPS) cells, CDI is committed to providing you with the support and resources needed for successfully using our iCell products in your research. From technical support to training to making technical documents available online, our goal is to provide industry-leading support.

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Frequently Asked Questions

 

iCell Products

What products does CDI offer?

CDI develops and manufactures human cells derived from induced pluripotent stem (iPS) cells.  CDI's product portfolio includes:

  • iCell Cardiomyocytes
  • iCell Endothelial Cells
  • iCell Neurons
  • iCell Hepatocytes (coming in 2012)
  • MyCell Products

What are the benefits of using CDI's iCell products?

CDI's iCell products offer numerous advantages over existing cellular models.  Specifically, CDI's iCell products are:

  • Biologically relevant human cells that exhibit characteristic phenotypes and functions.
  • Highly pure (>95%) populations of human cells
  • Readily available in the quantities required for use in drug discovery and safety testing.
  • Supported with the necessary media, protocols, training and technical/application support to ensure customer success).

Do I need specialized training to use CDI's iCell products?

No specialized training is needed to use CDI's iCell products. However, it is critical that CDI's iCell products be handled as described in the User's Guides. In addition, CDI offers training videos, technical/application support, and hands-on iCell Certification Programs to ensure customer success.

 

iCell Cardiomyocytes

Do iCell Cardiomyocytes consist of ventricular, atrial and/or nodal cardiomyocytes?

iCell Cardiomyocytes are a pan-cardiac population of ventricular, atrial, and nodal cells.  Based on CDI's stringent criteria for classifying cardiac subtypes, iCell Cardiomyocytes consist of approximately 50% ventricular, 25% atrial, and 25% nodal cells.

How pure are iCell Cardiomyocytes?

iCell Cardiomyocytes are >95% pure.

Are iCell Cardiomyocytes shipped fresh or cryopreserved?

iCell Cardiomyocytes are shipped cryopreserved to ensure maximum flexibility for use in a wide range of assays and applications.

Do iCell Cardiomyocytes proliferate in culture?

iCell Cardiomyocytes are terminally differentiated cardiomyocytes and similar to native human cardiomyocytes, they do not proliferate.

Do iCell Cardiomyocytes recapitulate typical human cardiomyocyte behavior in culture?

iCell Cardiomyocytes are metabolically active and beat spontaneously in culture, enabling robust measurements of electrophysiology, contractility, energetics, and intracellular signaling in a species-specific manner using a variety of assays and platforms.

How long do iCell Cardiomyocytes show typical functionality in culture?

CDI has demonstrated stable cardiomyocyte gene expression for up to 120 days and has maintained stable, beating monolayers of iCell Cardiomyocytes for greater than 9 months.  iCell Cardiomyocytes are provided with enough media for two weeks of culture and additional Maintenance Media can be purchased separately for applications requiring longer-term culture.

What characterization has CDI performed on iCell Cardiomyocytes?

iCell Cardiomyocytes have been extensively characterized over many physiological attributes and functions including (but not limited to):

  • Morphology
  • Gene and protein expression
  • Electrophysiology
  • Contractility
  • Biochemical pathways
  • Response to known cardiotoxicants and pathological insults

For what applications can iCell Cardiomyocytes be used?

iCell Cardiomyocytes have demonstrated utility in a wide variety of  applications including (but not limited to):

  • General cardiomyocyte biology
  • Toxicology
  • Safety pharmacology
  • Drug discovery
  • Disease research

What assays/platforms have iCell Cardiomyocytes been used with?

  • Perforated and whole cell manual patch clamp recording
    • Voltage clamp recording
    • Current clamp recording
  • Automated patch clamp recording
    • PatchXpress (Molecular Devices)
    • Patchliner (Nanion)
    • Qpatch (Sophion)
  • Multi-Electrode Array (MEA) recording
    • Multi-Channel Systems
    • Axion MEA (Axion Biosystems)
    • MED 64 (Alpha Med Scientific)
  • Contractility/GPCR Screening/Ca2+ handling
    • FLIPRTETRA GPCR and Ion Channel High Throughput Screening System (Molecular Devices)
    • Myocyte calcium and contractility recording system (Ionoptix)
    • Functional drug screening system (Hamamatsu)
  • High Content Analysis
    • ImageXpress (Molecular Devices)
    • Cellomics ArrayScan VTI (Thermo Scientific)
  • Label-Free Analysis
    • xCELLigence RTCA Cardio System (ACEA/Roche Applied Science)
  • Bioenergetics
    • Seahorse Extracellular Flux (XF) Analyzer (Seahorse Bioscience)
  • Cell Health and Viability Assays
    • CellTiter-Glo, Caspase-Glo, CytoTox, MultiTox Assays, GSH-Glo, HDAC-Glo (Promega)
    • Cell Meter JC-10 Mitochondrial Membrane Potential Assay (ABD Bioquest)
    • CyQUANT Direct Cell Proliferation Assay (Invitrogen)

Are there any publications citing the use of iCell Cardiomyocytes?

iCell Cardiomyocytes have been cited in numerous publications. Refer to the Published Research webpage.)

How do I thaw, culture, and use iCell Cardiomyocytes in my research?

CDI provides extensive product literature and technical support tools to enable the use of iCell Cardiomyocytes across a broad range of applications. Refer to the Product Literature webpage for a product User's Guide, Application Notes, Datasheets, and a training video. Hands-on training through CDI's iCertification Program is also available. Additional assistance can be obtained by contacting Technical and Application Support by completing the online form or by calling +1 (608) 310-5100 or (877) 310-6688 (US toll-free).

 

iCell Endothelial Cells

How pure are iCell Endothelial Cells?

iCell Endothelial Cells are >95% pure.

Are iCell Endothelial Cells shipped fresh or cryopreserved?

iCell Endothelial Cells are shipped cryopreserved to ensure maximum flexibility for use in a wide range of assays and applications.

Do iCell Endothelial Cells proliferate in culture?

Yes. iCell Endothelial Cells can be maintained in culture for up to 5 passages.

What characterization has CDI performed on iCell Endothelial Cells?

iCell Endothelial Cells have been characterized for the following:

  • Morphology
  • Gene and protein expression
  • Tube formation
  • Tight junction formation
  • Barrier function
  • Acetylated LDL uptake
  • Response to inflammatory stimuli

For what applications can iCell Endothelial Cells be used?

iCell Endothelial Cells can be used for the following applications:

  • Drug discovery
  • Oncology research
  • Inflammation research
  • Cardiovascular disease research

What assays/platforms have iCell Endothelial Cells been used with?

iCell Endothelial Cells have been used in a number of assays and platforms including:

  • Cell migration assays
    • Electrical Cell-substrate Impedence Sensing (ECIS) System (Applied Biophysics)
  • Barrier Function Assays
    • xCELLigence RTCA Cardio System (ACEA/Roche Applied Science)
    • Electrical Cell-substrate Impedence Sensing (ECIS) System (Applied Biophysics)
  • Bioenergetics
    • Seahorse Extracellular Flux (XF) Analyzer (Seahorse Bioscience)
  • Angiogenesis Assays
    • Endothelial Tube Formation Assay
    • Electrical Cell-substrate Impedence Sensing (ECIS) System (Applied Biophysics)

How do I thaw, culture, and use iCell Endothelial Cells in my research?

CDI provides extensive product literature and technical support tools to enable the use of iCell Endothelial Cells. Refer to the Product Literature webpage for a product User's Guide, Datasheet, application data, and poster presentation. Additional assistance can be obtained by contacting Technical and Application Support by completing the online form or by calling +1 (608) 310-5100 or (877) 310-6688 (US toll-free).

 

iCell Neurons

What population of cells do iCell Neurons consist of?

iCell Neurons are a mixture of post-mitotic neural subtypes, comprised of approximately equal parts of GABAergic and glutamatergic neurons and absent of astrocytes as measured by gene and protein expression.

How pure are iCell Neurons?

iCell Neurons are >95% pure.

Are iCell Neurons shipped fresh or cryopreserved?

iCell Neurons are shipped cryopreserved to ensure maximum flexibility for use in a wide range of assays and applications.

Do iCell Neurons proliferate in culture?

iCell Neurons are terminally differentiated neurons and similar to native human neuron. They do not proliferate.

Do iCell Neurons recapitulate typical human neurons behavior in culture?

iCell Neurons are metabolically active enabling robust measurements of electrophysiology, energetics, and intracellular signaling in a species-specific manner using a variety of assays and platforms.

How long do iCell Neurons show typical functionality in culture?

iCell Neurons display physiological characteristics and responses and a stable adherent single-cell morphology for an extended culture period (>14 days). iCell Neurons are provided with enough media for more than two weeks of culture. Additional Maintenance Media can be purchased separately for applications requiring longer-term culture.

What characterization has CDI performed on iCell Neurons?

iCell Neurons have been extensively characterized over many physiological attributes and functions including (but not limited to):

  • Morphology
  • Gene and protein expression
  • Electrophysiology
  • Biochemical pathways
  • Response to known neurotoxicants

For what applications can iCell Neurons be used?

iCell Neurons have demonstrated utility in a wide variety of  applications including (but not limited to):

  • General neurobiology
  • Toxicology
  • Drug discovery
  • Disease modeling

What assays/platforms have iCell Neurons been used with?

iCell Neurons have been used with a broad range of assays and platforms including (but not limited to):

  • Perforated and whole cell manual patch clamp recording
    • Voltage clamp recording
    • Current clamp recording
  • Automated patch clamp recording
    • PatchXpress (Molecular Devices)
    • Patchliner (Nanion)
  • Multi-Electrode Array (MEA) recording
    • Multi-Channel Systems
  • High Content Analysis
    • ImageXpress (Molecular Devices)
  • Cell Health and Viability Assays
    • CellTiter-Glo, Caspase-Glo, CytoTox, MultiTox Assays, GSH-Glo, HDAC-Glo (Promega)
    • Cell Meter JC-10 Mitochondrial Membrane Potential Assay (ABD Bioquest)
    • CyQUANT Direct Cell Proliferation Assay (Invitrogen)

How do I thaw, culture, and use iCell Neurons in my research?

CDI provides extensive product literature and technical support tools to enable the use of iCell Neurons across a broad range of applications. Refer to the Product Literature webpage for a product User's Guide, Datasheet, application data, and poster presentations. Additional assistance can be obtained by contacting Technical and Application Support by completing the online form or by calling +1 (608) 310-5100 or (877) 310-6688 (US toll-free).

Certificate of Analysis Lookup

To find your certificate of analysis, see the Certificate of Analysis Lookup page

COA Lookup

Training

CDI provides multiple training resources to fit your schedule and ensure your success working with iCell products:

Training




Note: See the full set of webinar videos on iCell products and other Cellular Dynamics technology, or subscribe to the Cellular Dynamics YouTube channel.

User Documentation

iCell Cardiomyocytes
iCell Cardiac Progenitor Cells
iCell Neurons
iCell DopaNeurons
iCell Astrocytes
iCell Hepatocytes
iCell Skeletal Myoblasts
iCell Endothelial Cells
iCell Hematopoietic Progenitor Cells
MyCell Products

iCell Cardiomyocytes

User Documentation

User's Guide: iCell Cardiomyocytes
(PDF)

Application Protocols

Dissociating Cardiomyocytes by Enzymatic Treatment
(PDF)

Extracting Total RNA
(PDF)

Immunofluorescent Labeling
(PDF)

New!

Measuring Cardiac Electrical Activity: Field Potential Detection with Axion Maestro Multielectrode Array
(PDF)

Measuring Cardiac Electrical Activity: Field Potential Detection with Multielectrode Array
(PDF)

Measuring Cardiac Activity: Impedance Detection with xCELLigence RTCA Cardio System
(PDF)

New!Measuring Cardiac Activity: Intracellular Calcium Flux Detection on the FLIPR Tetra System
(PDF)

Measuring Cardiac Electrical Activity: Manual Perforated Patch Clamp
(PDF)

Modeling Cardiac Hypertrophy: Endothelin-1 Induction with ELISA Analysis
(PDF)

Modeling Cardiac Hypertrophy: Endothelin-1 Induction with Flow Cytometry Analysis
(PDF)

Modeling Cardiac Hypertrophy: Endothelin-1 Induction with High Content Analysis
(PDF)

Modeling Cardiac Hypertrophy: Endothelin-1 Induction with qRT-PCR Analysis
(PDF)

Performing Bioenergetic Analysis: XF96 Extracellular Flux Analyzer
(PDF)

Using Transfection for siRNA Delivery
(PDF)

Safety Data Sheets (SDS)

iCell Cardiomyocytes
(PDF)

iCell Cardiomyocytes Plating Medium
(PDF)

iCell Cardiomyocytes Maintenance Medium
(PDF)

iCell Cardiac Progenitor Cells

Safety Data Sheets (SDS)

iCell Cardiac Progenitor Cells
(PDF)

iCell Neurons

User Documentation

User's Guide: iCell Neurons
(PDF)

Application Protocols

Assessing Neurite Outgrowth: Quantification with High Content Screening
(PDF)

Immunofluorescent Labeling
(PDF)

New!

Measuring Neuronal Activity: Extracellular Single-unit Recordings on the Maestro Multielectrode Array
(PDF)

Plating into 1536-well Cell Culture Plates
(PDF)

Silencing Gene Expression: siRNA Delivery by Transfection
(PDF)

 

Performing Bioenergetic Analysis: XF96 Extracellular Flux Analyzer
(PDF)

Safety Data Sheets (SDS)

iCell Neurons
(PDF)

iCell Neurons Maintenance Medium
(PDF)

 

iCell Neurons Medium Supplement
(PDF)

iCell DopaNeurons

User Documentation

User's Guide: iCell DopaNeurons
(PDF)

Safety Data Sheets (SDS)

iCell DopaNeurons
(PDF)

iCell DopaNeurons Maintenance Medium
(PDF)

iCell DopaNeurons Medium Supplement
(PDF)

iCell Nervous System Supplement
(PDF)

iCell Astrocytes

User Documentation

User's Guide: iCell Astrocytes Prototype
(PDF)

Safety Data Sheets (SDS)

iCell Astrocytes
(PDF)

iCell Hepatocytes

User Documentation

User's Guide: iCell Hepatocytes
Please contact Technical Support for this document

Application Protocol

Immunofluorescent Labeling
(PDF)

Safety Data Sheet

iCell Hepatocytes
HCC-100-010-001
(PDF)

iCell Skeletal Myoblasts

User Documentation

User's Guide: iCell Skeletal Myoblasts
(PDF)

Safety Data Sheets (SDS)

iCell Skeletal Myoblasts
(PDF)

iCell Endothelial Cells

User Documentation

User's Guide: iCell Endothelial Cells
(PDF)

Application Protocol

Assaying Barrier Function: xCELLigence RTCA Cardio System
(PDF)

Safety Data Sheets (SDS)

iCell Endothelial Cells
(PDF)

iCell Endothelial Cells Medium Supplement
(PDF)

MyCell Products

Application Protocol

New!

Collecting Blood and Isolating PBMCs
(PDF)

Safety Data Sheet

MyCell Products
(PDF)

iCell Hematopoietic Progenitor Cells

Safety Data Sheets (SDS)

iCell Hematopoietic Progenitor Cells
(PDF)