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iCell^® Neurons

iCell^® Neurons are derived from human induced pluripotent stem (iPS) cells and provide a unique in vitro system for preclinical drug discovery, neurotoxicity testing, predictive disease modeling, and basic cellular research. A better and more biologically relevant alternative to current cell models, iCell Neurons offer researchers access to commercial quantities of high quality, highly pure human neurons that possess typical phenotypic characteristics and functional of mature neurons.

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Advantages
Applications
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Literature

Specifications Cellular Dynamics International’s iCell Neurons are a highly pure population of human neurons derived from induced pluripotent stem (iPS) cells using CDI’s proprietary differentiation and purification protocols. iCell Neurons are a mixture of post-mitotic neural subtypes, comprised primarily of GABAergic and glutamatergic neurons, with typical physiological characteristics and functions. These cells quickly assume a typical neuronal morphology with branching neurites. In addition, iCell Neurons display a stable adherent single-cell morphology and remain viable for an extended culture period (≥14 days), making them amenable to a variety of electrophysiology, neurotoxicity, and synaptic neurotransmission assays. Easy to implement and available in commercial quantities, these high quality, highly pure neurons are shipped as cryopreserved suspensions of dissociated cells with specifically formulated culture media. Once thawed, iCell Neurons remain viable for extended culture periods, allowing for acute and chronic studies. iCell Neurons, Post-thaw, Represent a Highly Pure Population of Neurons and Extensive Neurite Networks

Cell Type	Neurons

Catalog #	NRC-100-010-001 (1 unit per vial) (Request a quote)

Organism	Human

Source	Differentiated from a CDI reprogrammed human iPS cell line

Quantity	>2.5 M platable cells per unit

Shipped	Frozen

Storage	Liquid nitrogen

Growth Properties	Adherent

Media	iCell Neurons Maintenance Medium, 100 ml iCell Neurons Medium Supplement, 2 ml Media supplied with order; quantities sufficient for more than 2 weeks of cell culture

Advantages

Human Cells	iCell Neurons are terminally differentiated from human iPS cells that provide an easily accessible and biologically relevant model system to more accurately predict the relevant in vivo human response for preclinical drug discovery, neurotoxicity testing, predictive disease modeling, and basic cellular research..

Highly Pure Cell Population	Provides neuron-specific responses to reference molecules.

Homogenous and Reproducible	iCell Neurons are available in sufficient homogenous quantities and demonstrate typical human neuron functions and responses, and are highly amenable for carrying out reproducible dose and time experiments.

Fully Functional Model	iCell Neurons exhibit standard biochemical characteristics of normal human neurons, and demonstrate utility in a variety of assays including electrophysiology, neurotoxicity, and synaptic neurotransmission assays.

Easy to Implement	Cells are shipped as cryopreserved suspensions of dissociated cells with iCell Neurons Maintenance Medium and iCell Neurons Medium Supplement, specially formulated for optimal cell performance. Simply thaw, mix and use.

Acute and Longer-term Testing	iCell Neurons remain viable in culture for at least 2 weeks, thus enabling assessment of both acute and longer-term testing.

iPS Cell-derived	iCell Neurons are differentiated from iPS cells reprogrammed from a non-embryonic terminally differentiated cell type, thus avoiding the controversial and ethical issues surrounding embryonic stem cell use.

Applications

iCell Neurons perform well in a wide variety of including (but not limited to):

Cell-based Assays

Apoptosis
ATP production
Cell viability
Oxidative stress
Mitochondrial dysfunction
Neurite outgrowth/sprouting

Electrophysiological Applications

Conventional patch clamping
Microelectrode (MEA) recording

Figure1: iCell Neurons Show a Cytotoxicity Dose Response to Known Compounds
iCell Neurons were cultured for 7 - 14 days post-thaw and exposed to a dilution series of (A) staurosporine and (B) kainic acid. Viability (as measured using cellular ATP content) was determined using the CellTiter-Glo^® Luminescent Cell Viability Assay. (Promega Corporation)

Figure 2: iCell Neurons Exhibit Neuron-like Action Potentials
Evoked and spontaneous action potentials were recorded from an iCell Neuron (9 and 14 days, respectively) using a whole-cell patch clamp methodology. All action potentials demonstrate an overshoot of the depolarization phase above 0 mV and an undershoot of the repolarization phase below baseline before correction to steady-state.

Figure 3: iCell Neurons Respond to Ion Channel Blockers
The conventional perforated patch voltage clamp technique was used with individual iCell Neurons to record the effects of compound application. The addition of classical neuron ion channel antagonists tetrodotoxin (TTX), tetraethylammonium (TEA) and nifedipine effectively blocked (A) inward sodium, (B) outward potassium, and (C) inward calcium currents, respectively, in iCell Neurons 12 - 13 days post-thaw.

To Order

Request a quote or contact Cellular Dynamics:
+1 (608) 310-5100 | US toll-free +1 (877) 310-6688

In Japan, contact IPS Academia Japan:
+81 75 256-8582

	Units per Vial	Catalog #

iCell Neurons* (includes iCell Neurons Maintenance Medium and Medium Supplement)	1	NRC-100-010-001

iCell Neurons Maintenance Medium		NRM-100-121-001

iCell Neurons Medium Supplement		NRM-100-031-001

* >2.5 M platable cells per unit

Support

Training	Prior to storing, thawing, seeding, plating, and maintaining iCell Neurons, read the iCell Neurons User's Guide for proper handling techniques. In-lab training may be available upon request.

Frequently Asked Questions	See the list of FAQs here.

Technical Support	Call +1 (608) 310-5100 \| US toll-free (877) 310-6688 or submit your technical question online.

Literature

Reference Materials
User Documentation
Technology Overview

Reference Materials


Datasheet	iCell Neurons (PDF)

Application Notes	Applying Transfection Technologies to Create Novel Screening Models (PDF)

Presentations	Induced Pluripotent Stem Cell-derived Tissues and their Role in Developing Novel Assays for Drug Discovery (PDF, presented at AIMBE 2013)

	Application of iPS Cell-derived Cells and a Novel Electrophysiology Platform for Neuronal and Cardiac Toxicity Evaluation and Drug Screening (PDF, presented at SLAS2013)

	Advanced Assays for In Vitro Toxicity Evaluation and Phenotypic Screening (PDF, presented at SLAS2013)

	Advancements in the Use of iPS Cell-derived Cells for In Vitro Disease Modeling and Phenotypic Screening (PDF, presented at SLAS2013)

	Characterizing Human iPS Cell-derived Neuronal Cultures: Expression and Function of Ion Channels and Receptors (PDF, presented at SLAS2012)

	Novel Assays for Drug Discovery and Toxicology Using Human iPS Cell-derived Neurons and Cardiomyocytes (PDF, presented at SLAS2012)

Bibliography	Published Research (HTML)

Posters	Application of Human iPS Cell-derived Models for Highly Predictive Toxicity Screening (PDF, presented at ELRIG 2013)

	Development and Characterization of Human Induced Pluripotent Stem Cell-Derived Model Systems for Muscular Dystrophy Disease Modeling and Drug Development (PDF, presented at the MDA 2013 Conference)

	Applications of Human iPSC-derived Neurons Using High-content Image-based Assays (PDF, presented at Society for Neuroscience Annual Meeting 2012)

	Development and Characterization of Human iPSC-derived Neurons for Drug Discovery Applications (PDF, presented at Society for Neuroscience Annual Meeting 2011)

	High Content Neuronal Toxicity Assays Using iPS Cell-derived Neurons (PDF, presented at SLAS2012)

	Evaluation of Network Electrophysiology for Neurotoxicity Screening (PDF, presented at Society for Neuroscience Annual Meeting 2012)

	Optimization of Neuronal Cultures Derived from Human Induced Pluripotent Stem Cells for High Throughput Assays of Synaptic Function (PDF, presented at ISSCR 2012)

	Applications Development at CDI: Improving Workflows, Pushing Biology, and Enabling Screening (PDF, presented at the CDI User Group Meeting 2012)

	Human iPSC-derived Cells for Modelling Cellular Bioenergetics: Building a Metabolic Profile Using the XF Mito Stress Test (PDF, presented at the CDI User Group Meeting 2012)

	Advancements in the Use of iPS Cell-derived Systems for In Vitro Disease Modeling and Phenotypic Screening (PDF, presented at StemCONN 2013)

	Implementation of Human iPSC-derived Cell Types Into High Throughput Screening Workflows (PDF, presented at SLAS 2013)

User Documentation


User's Guide	iCell Neurons (PDF)

Application Protocols	Assessing Neurite Outgrowth Using High Content Screening (PDF)

	Immunofluorescent Labeling (PDF)

	Multielectrode Array System Application Protocol (PDF)

	Plating into 1536-well Cell Culture Plates (PDF)

	Silencing Gene Expression: siRNA Delivery by Transfection (PDF)

	Performing Bioenergetic Analysis: XF96 Extracellular Flux Analyzer (PDF)

Material Safety Data Sheets (MSDS)	iCell Neurons (PDF)

	iCell Neurons Maintenance Medium (PDF)

	iCell Neurons Medium Supplement (PDF)

Technology Overview


Datasheet	Cellular Dynamics International: True Human Biology in a Dish (PDF)

Poster	Reprogramming Human Peripheral Blood Cells (PDF, presented at ISSCR 2009)

Bibliography	Published Research (HTML)

iCell® Neurons

Specifications