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iCell^® Endothelial Cells

CDI now provides iCell^® Endothelial Cells, interior surface blood vessel cells derived from human induced pluripotent stem (iPS) cells. iCell Endothelial Cells are specifically designed for use in vascular-targeted drug discovery, tissue regeneration, and other life science research.

iCell Endothelial Cells are highly purified human endothelial cells derived from iPS cells through CDI's proprietary differentiation and purification protocols. iCell Endothelial Cells exhibit characteristic endothelial gene and protein expression (e.g., CD31, CD105, CD144, ZO-1, and von Willebrand Factor) and endothelial cell functions (e.g., tubular formation, acetylated LDL uptake, barrier function, and wound healing). These cells are an ideal in vitro model system that provides a reliable source of endothelial cells suitable for use in vascular biology research including angiogenesis, atherosclerosis, inflammation, and many other research areas.

Data provided by Michael Schwartz, Ph.D. and Prof. William Murphy, University of Wisconsin-Madison.

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Specifications Cellular Dynamics International's iCell Endothelial Cells are highly purified human cells derived from iPS cells through CDI's proprietary differentiation and purification protocols. iCell Endothelial Cells exhibit characteristic endothelial gene and protein expression (e.g., CD31, CD105, CD144, ZO-1 and von Willebrand Factor) and endothelial cell functions (e.g., tubular formation, acetylated LDL uptake, barrier function and wound healing). These endothelial cells are an ideal in vitro model system, providing a reliable source of cells suitable for use in vascular biology research including angiogenesis, atherosclerosis, inflammation, and many other research areas. iCell Endothelial Cells Express von Willebrand Factor iCell Endothelial Cells were immunostained for von Willebrand Factor, a characteristic marker of endothelial cells. (A) Brightfield image of endothelial cells, 40X magnification. (B) Characteristic Weibel-Palade body staining is observed for vWF (green), and the nuclei are counterstained with Hoechst (blue), 40X magnification.

Cell Type	Endothelial cells

Catalog #	ECC-100-010-001 (1 unit per vial) (Request a quote)

Organism	Human

Source	Differentiated from a CDI reprogrammed human iPS cell line

Quantity	>1.0M viable cells per unit

Shipped	Frozen

Storage	Liquid nitrogen

Growth Properties	Adherents

Media	iCell Endothelial Cells Medium Supplement (supplied with order) VascuLife VEGF Medium Complete Kit* (order separately from Lifeline Cell Technologies, Cat. # LL-0003)

* To ensure high growth and purity, it is imperative to combine VascuLife VEGF Medium and iCell Endothelial Cells Medium Supplement to produce an optimized Maintenance Medium. Order the VascuLife VEFG Medium from LifeLine Cell Technologies.

Advantages


Human Cells	iCell Endothelial Cells are differentiated from human pluripotent stem cells that provide an easily accessible and physiologically relevant model system for vascular-targeted drug discovery, tissue regeneration and other vascular biology research.

Highly Pure Cell Population	Provides endothelial cell-specific responses to reference molecules.

Homogenous and Reproducible	iCell Endothelial Cells are available in sufficient homogenous quantities and demonstrate typical human endothelial cell behavior and responses, and are highly amenable for carrying out reproducible dose and time experiments.

Fully Functional Model	iCell Endothelial Cells exhibit standard biochemical characteristics of normal human endothelial cells, and demonstrate utility in a variety of assays including cell migration, cytokine stimulation, tubular formation, and impedance/barrier function.

Easy to Implement	Cells are shipped as cryopreserved suspensions of dissociated cells with iCell Endothelial Cells Medium Supplement, specially formulated to enhance basal medium for optimal cell performance. Simply thaw and use.

Acute and Longer-term Testing	iCell Endothelial Cells remain viable in culture for up to 5 passages, thus enabling assessment of both acute and longer-term testing.

iPS Cell-derived	iCell Endothelial Cells are differentiated from iPS cells reprogrammed from a non-embryonic terminally differentiated cell type, thus avoiding the controversial and ethical issues surrounding the use of embryonic stem cells.

Applications

iCell Endothelial Cells are amenable to a wide array of assays including:

Cell adhesion
Cell invasion and migration
Cell permeability
Cell proliferation
Cell viability
Impedance/transendothelial electrical resistance
Tubular formation

Figure 1: iCell Endothelial Cells Display Typical Tubule Formation Properties
iCell Endothelial Cells exhibit the capacity to form tubes in a thick layer of Matrigel (A). The same cells were also (B) immunostained for ULEX (UEA-1) (green), which selectively binds to the surface glycoproteins of endothelial cells. Tube formation and binding of ULEX are characteristics typical of endothelial cells.

Figure 2: iCell Endothelial Cells Demonstrate Endothelial Barrier Functions
To assess the barrier function, iCell Endothelial Cells were (A) immunostained for the tight junction protein ZO-1(green), and (B) resistance levels were quantified by measuring the transcellular electrical resistance (TER). The baseline TER value for iCell Endothelial Cells is shown in black. The addition of thrombin (red) disrupted the barrier function of the cells, thus decreasing the resistance, and cells were able to recover after a short period of time.

Figure 3: iCell Endothelial Cells Exhibit Adhesion Molecule Stimulation
iCell Endothelial Cells treated with 25 ng/ml TNFa for 24 hours showed increased stimulation of (A) CD54 and (B) CD146.

Figure 4: Migration and Wound Healing Responses to Agonists and Antagonists in iCells Endothelial Cells
iCell Endothelial Cells were grown to confluency and wounded with an electrical pulse using an ECIS instrument. (A) Migration/”healing” was greatly enhanced with the addition of VEGF, whereas (B) in the presence of SU1498, a VEGF inhibitor, there was no response to the addition of VEGF.

To Order

Request a quote or contact Cellular Dynamics:
+1 (608) 310-5100 | US toll-free +1 (877) 310-6688

In Japan, contact IPS Academia Japan:
+81 75 256-8582

	Units per Vial	Catalog #

iCell Endothelial Cells^ (includes iCell Endothelial Cells Medium Supplement*)	1	ECC-100-010-001

^ >1.0M viable cells per unit * To ensure high growth and purity, it is imperative to combine VascuLife VEGF Medium and iCell Endothelial Cells Medium Supplement to produce an optimized Maintenance Medium. Order the VascuLife VEFG Medium from LifeLine Cell Technologies (Cat. # LL-0003).

Support


Training	Prior to storing, thawing, seeding, plating, and maintaining iCell Endothelial Cells, read the iCell Endothelial Cells User's Guide for proper handling techniques. In-lab training may be available upon request.

Frequently Asked Questions	See the list of FAQs here.

Technical Support	Call +1 (608) 310-5100 \| US toll-free (877) 310-6688 or submit your technical question online.

Literature

Reference Materials
User Documentation
Technology Overview

Reference Materials


Datasheet	iCell Endothelial Cells (PDF)

Application Notes	Applying Transfection Technologies to Create Novel Screening Models (PDF)

	iCell Endothelial Cells - Assaying Cell Proliferation (PDF)

	iCell Endothelial Cells - Assaying Barrier Function (PDF)

Poster	Synthetic Extracellular Matrix for Investigating 3D Vascular Network Formation (PDF, presented at the Society for Biomaterials 2013)

	Application of Human iPS Cell-derived Models for Highly Predictive Toxicity Screening (PDF, presented at ELRIG 2013)

	Applications Development at CDI: Improving Workflows, Pushing Biology, and Enabling Screening (PDF, presented at the CDI User Group Meeting 2012)

	Human iPSC-derived Cells for Modelling Cellular Bioenergetics: Building a Metabolic Profile Using the XF Mito Stress Test (PDF, presented at the CDI User Group Meeting 2012)

	Advancements in the Use of iPS Cell-derived Systems for In Vitro Disease Modeling and Phenotypic Screening (PDF, presented at StemCONN 2013)

	Implementation of Human iPSC-derived Cell Types Into High Throughput Screening Workflows (PDF, presented at SLAS 2013)

User Documentation


User's Guide	iCell Endothelial Cells (PDF)

Application Protocol	Assaying Barrier Function: xCELLigence RTCA Cardio System (PDF)

Material Safety Data Sheets (MSDS)	iCell Endothelial Cells (PDF)

	iCell Endothelial Cells Medium Supplement (PDF)

Technology Overview


Datasheet	Cellular Dynamics International: True Human Biology in a Dish (PDF)

Poster	Reprogramming Human Peripheral Blood Cells (PDF, presented at ISSCR 2009)

Bibliography	Published Research (HTML)

iCell® Endothelial Cells

Specifications